Studies of stability and characterization this enzyme bromelain in pineapple (Ananas comosus)
نویسندگان
چکیده
Background Bromelain is the generic name given to the set of derived endopeptidases belonging to members of the Bromeliaceae family, which belongs to the pineapple (Ananas comosus), being able to break the peptide bond, separating proteins and amino acids [1]. Bromelain possesses a wide range of therapeutic benefit as property of facilitating digestion of proteins, meat softening, ability to facilitate blood clotting [2] And economic importance related to the food industry and textiles and production of drugs resulting in an increase of its value [3]. Thus, the aim of this work is to evaluate the stability of the enzyme in relation to different temperatures and pH to make feasible the purification of the same.
منابع مشابه
Structure prediction of Stem Bromelain from pineapples (Ananas Comosus) using procaricain enzyme as a modelling template
Bromelain is a general name for a family of sulfhydryl that can be found in the proteolytic enzyme group from pineapples (Ananas Comosus). In this study focuses on the prediction of three dimensional structure (3D) of bromelain using homology modelling by MODELLER. The amino acid sequence of bromelain was obtained from the NCBI database and the analysis was done using various bioinformatics too...
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Pineapple (Ananas comosus), Bromeliaceae family, is a fruit grows in tropical countries including Malaysia. This fruit has several pharmacological benefits due to the presence of high concentration of bromelain (cysteine proteases). Condition of elevated temperature will induce deformation of enzyme and result in loss of activity. Sulfhydryl groups in cysteine proteases are readily to be oxidiz...
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Background Allergies represent a socioeconomic problem that has affected increasing world population. A range of respiratory allergens are themselves proteases or are associated with proteolytic activity. Bromelain is a mixture of cysteine proteases present in the leaves and fruit Ananas comosus, with several pharmacological applications and is widely used in the food industry. Cases of occupat...
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Background Bromelain is a collective name for proteolytic enzymes or proteases (natural proteolytic enzymatic complex) found in tissues including stems, fruit and leaves of the pineapple (Ananas comosus var. comosus) and of other plant species of the family Bromeliaceae [1]. Is a bioactive agent possessing remarkable therapeutic properties such as reversible inhibition of platelet aggregation, ...
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Bromelain is a crude extract from the fruit or stem of pineapple [Ananas comosus (Linn.) Merr.] plant. It consists of different closely related proteinases which are good antiinflammatory, antithrombotic and fibrinolytic agents. The active fractions have been characterized biochemically and found to be effective after oral administration. It has earned universal acceptability as a phytotherapeu...
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